There are a wide variety of experiments that can be performed using flow cytometry.1) Experiment using fluorescent antibodiesAs we have already covered to some extent when introducing flow cytometry, the main purpose of flow cytometry is to find out and analyze what kind of cell it is by analyzing various proteins expressed by the cell. Therefore, many fluorescent antibodies that can specificall..

Principle of FluorescenceFluorescent dyes have wavelengths of light absorbed by each dye.If you shine light in the corresponding wavelength range of light, you can excite electrons in fluorescent dye.Excited electrons go to a higher energy state, an excited state, and then go back to a lower energy state, a ground state.At this time, the energy emitted is emitted in the form of photons.Through t..

Sample PreparationTissue fixation and permeabilization are the two most important aspects of sample preparation for FISH, significantly impacting the quality of the data extracted. Fixation is commonly achieved using 4% paraformaldehyde (PFA), which should be prepared fresh for each usage as PFA in solution forms polymers with long-term storage. An alternative is to use a 1:10 dilution of formal..

1. Studying Gene Function Experiment Choice: Gene Knockout, Knockdown, Overexpression, CRISPR-Cas9 Description: To understand the role of a specific gene in cells or organisms, direct gene manipulation methods are used. For example, to examine if the p53 gene suppresses tumors, researchers can create a p53 knockout mouse model to observe changes in cancer incidence. Selection Criteria: Choose kn..

Protein quantification is the process of determining the amount or concentration of protein contained in a sample and is a basic process for conducting experiments.In order to measure the amount of protein, an appropriate method must be selected and implemented based on the nature of other components contained in the sample as well as the protein, the speed and precision of quantitative analysis..

Protein-protein interactionsYeast Two-Hybrid SystemProtein Fragment Complementation AssayCo-ImmunoprecipitationAffinity Purification and Mass SpectrometryProximity Ligation AssayProtein-DNA InteractionsChIP-on-chipChip-sequencingDamIDMicroscale ThermophoresisProtein-RNA InteractionsToeprinting AssayTCP-seq