https://m.youtube.com/watch?v=3Y2DYxiPFV4

Performing basic scRNA-seq​1. Separating live single cells from tissues of interest2. Capture as much RNA as possible by lysing isolated cells - usually using poly[T]-primer3. Transform poly[T]-primed RNA into cDNA with reverse transcriptase (adding adapter sequences for sequencing platforms used and barcoding tasks for cell origin - adding unique molecular identifieres (UMIs))4. cDNA amplificat..

1. WritersWriters are enzymes that add chemical modifications to RNA molecules. They establish the modification marks on RNA, such as methylation or acetylation.Examples of Writers:m6A Methyltransferases: Add m6A modifications.METTL3-METTL14 complex: Core m6A methyltransferase.WTAP: A cofactor that enhances m6A writing.m5C Writers:NSUN family proteins: Add m5C to tRNA, rRNA, and mRNA.Pseudouridi..

The metabolism of xenobiotics is often divided into three phases: modification, conjugation, and excretion. These reactions act in concert to detoxify xenobiotics and remove them from cells.

"The laws of nature, like thermodynamics or ATP processes, never use 100% of their energy. Even the best batting average is only 0.3. What truly matters is the ability to maintaining energy to pursue over time.""Let’s focus on doing our best in areas where effort and control are within reach.Those who are faithful in small tasks can accomplish greater ones, and whether it’s catching a rabbit or ..