Sample PreparationTissue fixation and permeabilization are the two most important aspects of sample preparation for FISH, significantly impacting the quality of the data extracted. Fixation is commonly achieved using 4% paraformaldehyde (PFA), which should be prepared fresh for each usage as PFA in solution forms polymers with long-term storage. An alternative is to use a 1:10 dilution of formal..

1. Studying Gene Function Experiment Choice: Gene Knockout, Knockdown, Overexpression, CRISPR-Cas9 Description: To understand the role of a specific gene in cells or organisms, direct gene manipulation methods are used. For example, to examine if the p53 gene suppresses tumors, researchers can create a p53 knockout mouse model to observe changes in cancer incidence. Selection Criteria: Choose kn..

Protein quantification is the process of determining the amount or concentration of protein contained in a sample and is a basic process for conducting experiments.In order to measure the amount of protein, an appropriate method must be selected and implemented based on the nature of other components contained in the sample as well as the protein, the speed and precision of quantitative analysis..

Protein-protein interactionsYeast Two-Hybrid SystemProtein Fragment Complementation AssayCo-ImmunoprecipitationAffinity Purification and Mass SpectrometryProximity Ligation AssayProtein-DNA InteractionsChIP-on-chipChip-sequencingDamIDMicroscale ThermophoresisProtein-RNA InteractionsToeprinting AssayTCP-seq

ROS is known to play an important role in cells, such as cell signaling, induction, cell death, and gene expression.There are various experimental methods using ROS, but among them, the method of measuring using a fluorescent probe is widely used.This time, we will introduce a method of measuring and analyzing the results of ROS generation by cell growth stimulation using DCF fluorescent materia..

Drug receptors are protein or molecular targets where drugs bind to produce effects in the body. These receptors are generally classified into four main types: 1. Ion Channel-linked Receptors These receptors are connected to ion channels, and when a drug binds, the ion channel opens or closes, regulating the flow of ions into and out of cells. This rapid response plays an essential role in nerve..