1. Studying Gene Function Experiment Choice: Gene Knockout, Knockdown, Overexpression, CRISPR-Cas9 Description: To understand the role of a specific gene in cells or organisms, direct gene manipulation methods are used. For example, to examine if the p53 gene suppresses tumors, researchers can create a p53 knockout mouse model to observe changes in cancer incidence. Selection Criteria: Choose kn..

Protein quantification is the process of determining the amount or concentration of protein contained in a sample and is a basic process for conducting experiments.In order to measure the amount of protein, an appropriate method must be selected and implemented based on the nature of other components contained in the sample as well as the protein, the speed and precision of quantitative analysis..

Protein-protein interactionsYeast Two-Hybrid SystemProtein Fragment Complementation AssayCo-ImmunoprecipitationAffinity Purification and Mass SpectrometryProximity Ligation AssayProtein-DNA InteractionsChIP-on-chipChip-sequencingDamIDMicroscale ThermophoresisProtein-RNA InteractionsToeprinting AssayTCP-seq

ROS is known to play an important role in cells, such as cell signaling, induction, cell death, and gene expression.There are various experimental methods using ROS, but among them, the method of measuring using a fluorescent probe is widely used.This time, we will introduce a method of measuring and analyzing the results of ROS generation by cell growth stimulation using DCF fluorescent materia..

DNA microarray: Microarray is a DNA chip in which very small DNA fragments are integrated on a solid surface: Simultaneously measure the expression level of a large number of genes and compare the differences in gene expression between groups*Analyze the expression patterns of multiple mRNAs transcribed from cells: Since many samples are tested simultaneously, a large amount of data can be obtai..

IntroGenetic reporting assay is an experimental technique that is widely used for cell response or gene expression research due to external stimulation in prokaryotic or eukaryotic cells.In particular, the dual-reporter assay uses two independent reporter systems to increase the accuracy of the experiment.One of them is an experimental reporter, whose response degree varies depending on the expe..