https://m.youtube.com/watch?v=3Y2DYxiPFV4

Performing basic scRNA-seq​1. Separating live single cells from tissues of interest2. Capture as much RNA as possible by lysing isolated cells - usually using poly[T]-primer3. Transform poly[T]-primed RNA into cDNA with reverse transcriptase (adding adapter sequences for sequencing platforms used and barcoding tasks for cell origin - adding unique molecular identifieres (UMIs))4. cDNA amplificat..

1. WritersWriters are enzymes that add chemical modifications to RNA molecules. They establish the modification marks on RNA, such as methylation or acetylation.Examples of Writers:m6A Methyltransferases: Add m6A modifications.METTL3-METTL14 complex: Core m6A methyltransferase.WTAP: A cofactor that enhances m6A writing.m5C Writers:NSUN family proteins: Add m5C to tRNA, rRNA, and mRNA.Pseudouridi..